Warning: Can't synchronize with repository "(default)" (/home/git/ome.git does not appear to be a Git repository.). Look in the Trac log for more information.
Notice: In order to edit this ticket you need to be either: a Product Owner, The owner or the reporter of the ticket, or, in case of a Task not yet assigned, a team_member"

Task #11784 (closed)

Opened 10 years ago

Closed 10 years ago

Last modified 10 years ago

Bug: CZI openBytes fetches incorrect pixel data

Reported by: omero-qa Owned by: mlinkert
Priority: minor Milestone: 5.0.2
Component: Bio-Formats Version: 4.4.9
Keywords: ZeissCZIReader Cc: ebbwdan@…, martin.huettinger@…
Resources: n.a. Referenced By: n.a.
References: n.a. Remaining Time: n.a.
Sprint: n.a.

Description (last modified by rleigh)

https://www.openmicroscopy.org/qa2/qa2/qa/feedback/7748/

For big planes (~10000x10000 in this case), it looks like we are miscalculating the offsets in the CZI reader; this results in odd banding and wrapping of the image. I have attached an image of the misrendered plane to illustrate.

I have put in /ome/team/rleigh/czi:

  • IP-Stitching-04.czi (original file; opens fine with Zen Blue 2012)
  • IP-Stitching-04-zenblue.ome.tiff (zen blue exported ome-tiff; uses 2010-06 schema and the metadata is incorrect, but it opens and displays correctly)
  • IP-Stitching-04-bfconv.ome.tiff (original file converted to ome-tiff with bfconvert; valid pixels is 16 bit, but should really be 12; could the CZI reader be updated to set the valid pixels correctly?--it's correct for the CZI image)

Attachments (2)

Screen shot 2013-12-04 at 11.53.59.png (720.7 KB) - added by rleigh 10 years ago.
Screen shot 2013-12-04 at 12.15.00.png (327.0 KB) - added by rleigh 10 years ago.

Download all attachments as: .zip

Change History (10)

Changed 10 years ago by rleigh

comment:1 Changed 10 years ago by rleigh

  • Component changed from from QA to Bio-Formats
  • Description modified (diff)

Changed 10 years ago by rleigh

comment:2 Changed 10 years ago by mlinkert

  • Owner changed from mlinkert-x to mlinkert

comment:3 Changed 10 years ago by mlinkert

comment:4 Changed 10 years ago by rleigh

See also: QA #7771 and the files in /ome/team/rleigh/7771 which are the original QA upload plus additional information (export of the image from zen so you can see how it should look, plus some metadata from zen about the image size and pixel type and tile setup in zen.txt).

comment:5 Changed 10 years ago by rleigh

  • Cc martin.huettinger@… added

comment:6 Changed 10 years ago by mlinkert

  • Milestone changed from Unscheduled to 5.0.2

comment:7 Changed 10 years ago by mlinkert

  • Resolution set to fixed
  • Status changed from new to closed

Fixed with https://github.com/openmicroscopy/bioformats/pull/1078; email forthcoming on ome-users/ome-devel with details of how to try this out prior to 5.0.2.

comment:8 Changed 10 years ago by rleigh

The images now all look fine, thanks. For #7771 it would be nice if we could handle the image as a single series but that will need tiling support in bioformats.

Note: See TracTickets for help on using tickets. You may also have a look at Agilo extensions to the ticket.

1.3.13-PRO © 2008-2011 Agilo Software all rights reserved (this page was served in: 0.85879 sec.)

We're Hiring!